Volatile Anesthetics Bierentially Affect Irnmunostimulated Expression of inducible Nitric Oxide Syntbase
نویسنده
چکیده
Bac&ground: Nitric oxide released by inducible nitric oxide synthase (WOS) plays an important role in immune responses and systemic vasodilation in septic shock. Volatile anesthetics have been reported to interfere with signal transduction and gene expression. We studied the effect of volatile anesthetics on activity and expression of iNOS and potential mechanisms of action. Methods: Nitrite release and iNOS expression were determined using the Griess reaction and Western and Northern blot and protein levels of iNOS and NOS activity after a single stimulation with lipopolysaccharide or y-interferon, most likely by attenuating intracellular calcium increase. Costimulation with lipopolysaccharide plus y-interferon, however, results in maximum WOS expression and activity, which are no longer inhibited but are potentiated by volatile anesthetics by unidentified mechanisms. (
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